LAP Lambert Academic Publishing 9783659563287 光度计-产品快照
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LAP Lambert Academic Publishing 9783659563287 光度计
Multicomponent analysis using UV-visible spectrophotometer - Hardcover, Softcover - Language: eng
LAP Lambert Academic Publishing
Artikel-Nr.: 858C-9783659563287 Hersteller: LAP Lambert Academic Publishing Herst.-Nr.: 9783659563287 EAN/GTIN: 9783659563287 Multicomponent analysis using UV-visible spectrophotometer In the present work, a simple, cost effective, reproducible and reliable method for simultaneous estimation of Glimepiride and Pioglitazone-HCl in bulk and marketed formulation using simultaneous equation was developed and validated. The developed method involved measurement of absorbances at wavelength of 228 nm and 265.5 nm ( absorption maxima of Glimepiride and Pioglitazone-HCl respectively), which these obeyed the principle of simultaneous equation method. Both the drugs obeyed Beer-Lambert s law in concentration ranges (For Glimepiride 4-20 and for Pioglitazone-HCl 10-50 mcg/ml). The validation parameters studied according to I.C.H Q2B guidelines.The recovery studies (found to be 100.3 % and 100.01 % respectively). The molar absorptivity and Sandell s sensitivity values show the sensitivity of the method. The values of coefficient of variance suggest high level of precision of the method.The LOD and LOQ value for Glimepiride were 0.0161 and 0.0486 mcg/ml and of Pioglitazone-HCl were 0.213 and 0.645 g/ml respectively. The developed and validated method can successfully be applicable for the estimation of drug content in various marketed formulations. Weitere Informationen: Autor: Jain, Suresh Verlag: LAP Lambert Academic Publishing Sprache: eng
Autor: Jain, Suresh Verlag: LAP Lambert Academic Publishing Sprache: eng
Startseite Medizinbedarf, Therapie, Labor Laborbedarf Optische Untersuchung Photometer Spektralphotometer
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