GTX80745-产品快照
- 品牌:
- GTX80745
- 型号:
- GTX80745
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产品介绍
GTX80745
Genetex - GTX80745 - NPY Y2 antibody - 50µl (Each (50µl))
GTX80745
This NPY Y2 antibody has been extensively characterized at the spinal level and is currently being characerized in brain.? The antibody has been tested in both rat and mouse and appears to work better on mose tissue, although positive staining is seen in rat.? For full characterizarion in dorsal root ganglion and spinal cord see: J Comp Neurol. 2005 Aug 29;489(3):328-48. This listing is for Each (50µl) See other products in some related categories (click to browse): Industrial & Scientific > Laboratory & Science Antigen NPY Y2 Host Rabbit Reactivity Mouse, Rat Isotype IgG Unit Each (50µl) Application Note For IHC: Use at a dilution of 1:500 -1:1, 000. Not tested in other applications. Optimal dilutions/concentrations should be determined by the researcher. Antiserum was used on perfusion fixed tissue. Perfusion: 1) calcium-free Tyrode's solution, 2) paraformaldehyde-picric acid fixative, and 3) 10% sucrose in PBS as a cryo-protectant. Desired tissues were dissected and stored overnight in 10% sucrose in PBS. Slide-mounted tissue sections were processed for indirect immunofluorescence. Slides were incubated with blocking buffer for 1 hour at room temperature. Primary antiserum was diluted with blocking buffer to the appropriate working concentration. Blocking buffer was removed and slides were incubated for 18-24 hours at 4oC with primary antiserum. Slides were rinsed 3 times and then incubated with secondary antibodies for 1 hour at room temperature. Slides were again rinsed 3 times and coverslipped. Staining was examined using fluorescence microscopy. ChIP No ELISA No Flow Cytometry No Gel Shift No Immunochemistry Yes Immunofluorescence No Immunoprecipitation No Monoclonal/Polyclonal Polyclonal Neutralization No Storage Buffer Phosphate-buffered saline containing 0.05% sodium azide Storage Instruction Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. Tested Applications IHC Type Primary Western Blot No
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