GTX30112-产品快照
- 品牌:
- GTX30112
- 型号:
- GTX30112
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产品介绍
GTX30112
Genetex - GTX30112 - DNA polymerase epsilon antibody [3C5.1] (Each (50µl))
GTX30112
DNA polymerase e from HeLa cells differs from yeast pol e in that HeLa pol e contains two subunits of 220 and 55 kDa, whereas yeast pol e has one large subunit and three smaller subunits. Previous studies of template specificity of HeLa pol e, which appears to be required for long patch DNA repair in permeabilized cells, indicate pol e prefers long stretches of single-stranded regions in poly(dA)-oligo(dT) as template. HeLa pol a and pol d preferactivated DNA and alternating poly(dA-dT) as templates, respectively. Pol e retains its processivity in the absence of proliferating cell nuclear antigen (PCNA), unlike pol d. PCNA can also interact with Pol e on linear DNA templates, even in the absence of other auxiliary factors (replication factor C, replication factor A), and thereby stimulate its primer recognition and DNA synthesis. This listing is for Each (50µl) See other products in some related categories (click to browse): Industrial & Scientific > Laboratory & Science Antigen DNA Polymerase epsilon Host Mouse Reactivity Hamster, Human, Mink, Monkey, Mouse Clone 3C5.1 Isotype IgG2a Unit Each (50µl) Application Note For WB: Use at a concentration of 5 ug/ml. This antibody can be used in Western blot analysis and detects a single band of 220 kDa representing the catalytic subunit of DNA Pol epsilon. Product GTX30112 will immunoprecipitate, but only in the absence of salt and even then poorly. Not tested in other applications. Optimal dilutions/concentrations should be determined by the researcher. ChIP No ELISA No Flow Cytometry No Gel Shift No Immunochemistry No Immunofluorescence No Immunogen Mono S FPLC column purified HeLa cell pol epsilon. Immunoprecipitation No Monoclonal/Polyclonal Monoclonal Neutralization No Purification Affinity purified Storage Instruction Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. Tested Applications WB Type Primary Western Blot Yes
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