GTX28289-产品快照
- 品牌:
- GTX28289
- 型号:
- GTX28289
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产品介绍
GTX28289
Genetex - GTX28289 - Cardiac Troponin I antibody [19C7] (Each (200µG))
GTX28289
This antibody targets human Troponin I. This listing is for Each (200µG) See other products in some related categories (click to browse): Industrial & Scientific > Laboratory & Science Antigen Cardiac Troponin I Host Mouse Reactivity Cat, Cow, Dog, Fish, Goat, Human, Mouse, Pig, Rabbit, Rat Clone 19C7 Isotype IgG2b Unit Each (200µG) Application Note AP: Use at an assay dependent concentration. ELISA: Use at an assay dependent dilution. IA: Use at an assay dependent concentration. WB: Use at an assay dependent concentration. These antibody combinations are recommended for sandwich ELISA, and demonstrate high sensitivity (0.02 ng/ml), good kinetics, low background and high reproducibility: Can be paired for ELISA with GTX210226 Mouse monoclonal [16A11] to cardiac Troponin I or GTX210230 Mouse monoclonal [3C7] to cardiac Troponin I or GTX28290 Mouse monoclonal [22B11] to cardiac Troponin I . Can also be paired for ELISA with GTX210239 Mouse monoclonal [MF4] to cardiac Troponin I , or GTX210231 Mouse monoclonal [4C2] to cardiac Troponin I for capture, with GTX210226 Mouse monoclonal [16A11] to cardiac Troponin I and GTX28286 Mouse monoclonal [7B9] to cardiac Troponin C for detection. Can also be paired with GTX210236 Mouse monoclonal [M18] to cardiac Troponin I for capture, with GTX210239 Mouse monoclonal [MF4] to cardiac Troponin I and GTX210226 Mouse monoclonal [16A11] to cardiac Troponin I . Using multiple combinations of MAbs antibodies with different epitope specificity as capture and multiple MAbs as detection, the sensitivity of the sandwich ELISA can be increased 2-4 fold. The ELISA based on these recommended Abs, recognize complexed and free forms of the antigen with same sensitivity. For better sensitivity and reproducibility for assay designing we recommend to use at least one antibody that recognizes the stable fragment of the cTnI molecule (30-110 aa section), as cTnI is proteolytically degraded in the blood stream. Because the amount of complexed cTnI in blood of AMI patients is very high, for cTnI immunodetection we recommend using also a MAb that is specific to TnC or a MAb specific to TnC together with MAb(s) specific to cTnI. Such approach helps improving the sensitivity and reproducibility of cTnI immunoassays. Not tested in other applications. Optimal dilutions/concentrations should be determined by the end user. ChIP No ELISA Yes Flow Cytometry No Gel Shift No Immunochemistry No Immunofluorescence No Immunogen Native troponin complex Immunoprecipitation No Monoclonal/Polyclonal Monoclonal Neutralization No Purification Protein A purified Storage Buffer PBS with 0.1% sodium azide, pH 7.4 Storage Instruction Keep as concentrated solution, aliquot and store at 4°C. Do not freeze. We recommend to parafilm vials during storage to prevent evaporation of buffer. Tested Applications Apuri, ELISA, IA, WB Type Primary Western Blot Yes
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